Collect,ShipandPreserveDNAinWholeBloodatRoomTemperature

• PreserveandMaintainSampleIntegrityforupto14months
• CompatIBLewithdifferentextraction/purificationtechniques
• Compatiblewithalldownstreamapplications

Overview

DNAgard™BloodisdesignedfortheimmediatestABIlizationofDNAinbloodwiththeconvenienceofroomtemperatureshippingandstorage.ThepreservativereagentrapidlypermeatescellularstructuresandmembranestostabilizeandprotectgenomicDNAinwholebloodsamples.TheuseofDNAgardBloodallowsastreamlinedworkflowfrombloodcollectionintheclinicorfieldtosampleprocessinginthelaboratory.

• Collect,shipandpreserveDNAinbloodunderoptimalconditions
• RecoverhighqualityDNA(highMWfragments)
• RecoverquantitativeDNA(yield)

ForResearchUseOnly.Notforuseindiagnosticprocedures.

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ProductData

ProtectDNAatambienttemperatures

Figure1:DNAgardBloodformulationpreservesDNAinwholebloodfor14months.GelimagesofisolatedgenomicDNArecoveredfrombloodsamplespreservedfor14monthsinDNAgardBlood(DGB)atroomtemperature,inacompetitorpreservative(COM)atroomtemperature,unprotected(NP)atroomtemperature,versuscontrol(-20°C).

Broadrangeofapplications

DNAprotectedusingtheDNAgardBloodformulationissuitableforuseinmanydownstreamapplications,e.g.long-rangePCR,realtimePCR,etc.

Figure2A.SuccessfulLong-rangePCRafter2monthsstorage.GenomicDNAisolatedfromhumanwholebloodstoredfor60daysintheDNAgardBloodformulation,non-protected(NP)orfrozenat-20°C.Long-rangePCRamplificationofa22KbpampliconwasperformedontheDNA(M=1kbladder).Figure2B.Real-timePCRafter1monthstorage.GenomicDNAisolatedfromhumanwholebloodstoredfor30daysintheDNAgardBloodformulationorfrozenat-20°Cwasquantifiedusingreal-timePCRamplificationoftheb-actingene.

RepresentativeDNAyield

DNAprotectedusingtheDNAgardBloodformationissuitableforuseinmanydownstreamapplications,e.g.long-rangePCR,realtimePCR,etc.

Figure3.qPCRquantificationafter1monthstorageofblood,from3differentdonors,inDNAgardBloodformulation(RT),Competitor(RT)orfrozen(-20°C).

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Specifications

• Productformats:100mLliquidreagent
• Applicationvolume:1:4(vol:vol)ofstabilizer:blood
• DNARecovery:~99.9%
• BloodDNApreservation:Atleast14monthsatroomtemperature
• Usage:ForResearchUseOnly.Notforuseindiagnosticprocedures.

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