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Biomatrica/DNAgard™ Tissue/ 90028-290

Biomatrica/DNAgard™ Tissue/ 90028-290
  • Biomatrica/DNAgard™ Tissue/ 90028-290
商品介绍

Collect,ShipandStoreTissues&CellsatAmbientTemperature

  • ProtectDNAintissues,cellsandbiopsymaterialfromdegradationatroomtemperature
  • CompatIBLewithmostdownstreamapplications,e.g.,PCR,qPCRandsequencing.
  • Reducesstorageandshippingcostsandimproveslabspacemanagement

Overview

DNAgard™isdesignedforimmediatestABIlizationofDNAfromintactcellsandtissueswiththeconvenienceofroomtemperatureshipping,processingandstorage.TheliquidstoragereagentrapidlypermeatescellmembranestostabilizeandprotectgenomicDNA.

DNAgardWorkflowOverview

ForResearchUseOnly.Notforuseindiagnosticprocedures.

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ProductData

DNAprotectioninratkidneytissuesatroomtemperature

Figure1:GelimageshowinggenomicDNAisolatedfromratkidney(25mg)storedfor195daysinDNAgard,water(non-protected:NP)orfrozenat-80°Cwasanalyzedon0.8%agarosegel.(M=1kbladder).

DNAgardTissueprotectsDNAinavarietyoftissues

Figure2:GelimagesshowinggenomicDNAisolatedfromavarietyofrattissues(25mg)storedfor70daysinDNAgardTissue,water(non-protected:NP)orfrozenat-80°Cwasanalyzedona0.8%agarosegel.(M=1kbladder).

DNAgardprotectsDNAinmammaliancellsatroomtemperature

Figure3:(A)106293Tcellswerepelletedandthesupernatant(media)wasremoved.CellpelletswereresUSPendedin500µlofDNAgardorwater(non-protected).Forcomparison,106cellswerespottedonWhatmanFTApaperanddried.Asapositivecontrol,106cellswerestoredat–80°Cin10%DMSO.DNAgard,Whatmanandnon-protectedsampleswerestoredatroomtemperature.After84daysofincubationsampleswereprocessedforDNAisolationusingacommerciallyavailablecolumnpurificationtechnology.(TheDNAwaselutedfromtheWhatmanFTAsamplesusingWhatman’spublishedprotocol).TheDNAyieldandintegrityfrom5x104293Tcellswascompareddirectlyona0.8%agarosegel.(T’0′isDNArecoveredatthetimecellswereharvested;M=1kbladder).(B)RepresentativeDNAyieldsfromDNAgardsamplescomparedtofrozencontrols.1DNAwasisolatedfromtissuefragmentsor106mammalian293TcellsstoredinliquidDNAgardfor2months.DNAyieldwasquantifiedusingtheQuant-iTPicoGreenfluorescentassay(Invitrogen).YieldispresentedasngofDNApermgtissuefortheanimaltissuesamplesortotalDNAforthe293Tcellsamples.

Broadrangeofapplications

Successfulreal-timePCRandlong-rangePCRafter2monthsstorageinDNAgardandat-80°C

Figure4:Ratkidneyfragmentswereweighed(approx.25mgeach),immediatelysubmergedinDNAgardsolutionorwater(NP),andstoredatroomtemperature.Controlsampleswerefrozenandstoredat-80°C.After71days,totalDNAwasrecoveredfromthestoredsamplesusingacommerciallyavailablecolumnpurificationtechnology.DNAwasanalyzedbyrealtimePCRamplificationofthebeta-actingene.

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Specifications

  • Productformats:100mLbottle,Customvolumes
  • Applicationvolume:Atleast100µLper10mgtissue;100µLper106cells
  • DNAstorage:6months
  • DNAstabilizationtestingtemperature:20°C
  • Usage:ForResearchUseOnly.Notforuseindiagnosticprocedures.

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