Collect,ShipandStoreTissues&CellsatAmbientTemperature
- ProtectDNAintissues,cellsandbiopsymaterialfromdegradationatroomtemperature
- CompatIBLewithmostdownstreamapplications,e.g.,PCR,qPCRandsequencing.
- Reducesstorageandshippingcostsandimproveslabspacemanagement
Overview
DNAgard™isdesignedforimmediatestABIlizationofDNAfromintactcellsandtissueswiththeconvenienceofroomtemperatureshipping,processingandstorage.TheliquidstoragereagentrapidlypermeatescellmembranestostabilizeandprotectgenomicDNA.
DNAgardWorkflowOverview
ForResearchUseOnly.Notforuseindiagnosticprocedures.
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ProductData
DNAprotectioninratkidneytissuesatroomtemperature
Figure1:GelimageshowinggenomicDNAisolatedfromratkidney(25mg)storedfor195daysinDNAgard,water(non-protected:NP)orfrozenat-80°Cwasanalyzedon0.8%agarosegel.(M=1kbladder).
DNAgardTissueprotectsDNAinavarietyoftissues
Figure2:GelimagesshowinggenomicDNAisolatedfromavarietyofrattissues(25mg)storedfor70daysinDNAgardTissue,water(non-protected:NP)orfrozenat-80°Cwasanalyzedona0.8%agarosegel.(M=1kbladder).
DNAgardprotectsDNAinmammaliancellsatroomtemperature
Figure3:(A)106293Tcellswerepelletedandthesupernatant(media)wasremoved.CellpelletswereresUSPendedin500µlofDNAgardorwater(non-protected).Forcomparison,106cellswerespottedonWhatmanFTApaperanddried.Asapositivecontrol,106cellswerestoredat–80°Cin10%DMSO.DNAgard,Whatmanandnon-protectedsampleswerestoredatroomtemperature.After84daysofincubationsampleswereprocessedforDNAisolationusingacommerciallyavailablecolumnpurificationtechnology.(TheDNAwaselutedfromtheWhatmanFTAsamplesusingWhatman’spublishedprotocol).TheDNAyieldandintegrityfrom5x104293Tcellswascompareddirectlyona0.8%agarosegel.(T’0′isDNArecoveredatthetimecellswereharvested;M=1kbladder).(B)RepresentativeDNAyieldsfromDNAgardsamplescomparedtofrozencontrols.1DNAwasisolatedfromtissuefragmentsor106mammalian293TcellsstoredinliquidDNAgardfor2months.DNAyieldwasquantifiedusingtheQuant-iTPicoGreenfluorescentassay(Invitrogen).YieldispresentedasngofDNApermgtissuefortheanimaltissuesamplesortotalDNAforthe293Tcellsamples.
Broadrangeofapplications
Successfulreal-timePCRandlong-rangePCRafter2monthsstorageinDNAgardandat-80°C
Figure4:Ratkidneyfragmentswereweighed(approx.25mgeach),immediatelysubmergedinDNAgardsolutionorwater(NP),andstoredatroomtemperature.Controlsampleswerefrozenandstoredat-80°C.After71days,totalDNAwasrecoveredfromthestoredsamplesusingacommerciallyavailablecolumnpurificationtechnology.DNAwasanalyzedbyrealtimePCRamplificationofthebeta-actingene.
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Specifications
- Productformats:100mLbottle,Customvolumes
- Applicationvolume:Atleast100µLper10mgtissue;100µLper106cells
- DNAstorage:6months
- DNAstabilizationtestingtemperature:20°C
- Usage:ForResearchUseOnly.Notforuseindiagnosticprocedures.
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