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Biomatrica/DNAstāble™ / LD/ 90021-001/ 1 x 96-well plate

Biomatrica/DNAstāble™ / LD/ 90021-001/ 1 x 96-well plate
  • Biomatrica/DNAstāble™ / LD/ 90021-001/ 1 x 96-well plate
商品介绍

StABIlizestoreandshippurifiedDNAatroomtemperature

  • Long-termstabilization,storageandshipmentofDNAatroomtemperature
  • HighyieldDNArecoveryandusewithoutfurtherpurification
  • CompatIBLewithmostdownstreamapplications,e.g.,PCR,sequencing,andmicroarrays
  • Availableinavarietyofcompactformats,e.g.,tubes,multi-wellplates&custom
  • Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement

Product

DNAstāble™enablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatambienttemperatures.Samplerecoveryisachievedwithinminutesbysimplyaddingwaterorbuffertothematrix.Sampleisreadyfordownstreamanalysiswithoutneedforanyadditionalpurificationstep.

PropertiesBiomatricaUnprotectedColdChain
Yield
Stability
QualityofAmplification
LowConcentrationDNA
ShortTermStorage<1>
LongTermStorage>1year
ShippingRobustness
CostEffectiveness

DNAstabilizationcanbeachievedintwoformats:

Pre-driedintubesorplates(DNAstable)

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Data

SuccessfulPCRafterlongtermstorageinDNAstable:

Figure1.Aliquots(1ng)ofpDNA,eitherstoredinDNAstable(DS)orunprotected(NP)for26monthsatambienttemperature(RT)orat60°Cunderacceleratedagingconditions(equivalentto30yearsofroomtemperaturestorage)wereanalyzedbyPCRamplificationusingpUC19specificprimersgeneratinga490bpamplicon.ReferenceDNAsamples(1ng)werestoredat-20°C(control).

StabilityinLiquid-to-Dry(LD)Format:

Figure2.Twodifferentaliquots(20ng)ofgDNAstoredinDNAstable(DS)atroomtemperaturewithouthumidiycontrolfor3.5yearswereanalyzedbyPCRamplificationusingβ-actinspecificprimersgeneratinga500bpamplicon.ReferenceDNAsample(100ng)wasstoredat-20°C.

RecoveryofgenomicDNAafter26monthsofstorageinDNAstable

Figure3.GenomicDNAsampleswerestoredfor26monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control-20°C;StoredinDNAstable(DS)atroomtemperature(RT);Unprotected(NP)atroomtemperature;StoredinDNAstable(DS)fortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C);5)Unprotected(NP)andstoredfortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C).

qPCRofgenomicDNAstoredfor26monthsinDNAstable

Figure4.qPCRtracesofexperimentsdescribedinFigure3abovewithstandarddeviationdisplayed.

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Specifications

  • Productformats:
    • 1.7mltubesand96well-plates(dry)
    • 2mLand10mLbottles(liquid-to-dry)
    • Custom
  • Applicationvolume:5to150μl
  • Applicationamount:0.001to30μg
  • Dryingtime:10min(SpeedVac)toovernight(Laminarhood)
  • Recoveryvolume:10to100μl
  • Recovery:∼99.9%
  • DNAlong-termstorage:30yearsacceleratedaging;∼4years″realtime″
  • DNAstabilizationtestingtemperature:Temperature>60°C

Downloads

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