StABIlizestoreandshippurifiedDNAatroomtemperature

• Long-termstabilization,storageandshipmentofDNAatroomtemperature
• HighyieldDNArecoveryandusewithoutfurtherpurification
• CompatIBLewithmostdownstreamapplications,e.g.,PCR,sequencing,andmicroarrays
• Availableinavarietyofcompactformats,e.g.,tubes,multi-wellplates&custom
• Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement

Product

DNAstāble™enablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatambienttemperatures.Samplerecoveryisachievedwithinminutesbysimplyaddingwaterorbuffertothematrix.Sampleisreadyfordownstreamanalysiswithoutneedforanyadditionalpurificationstep.

DNAstabilizationcanbeachievedintwoformats:

Pre-driedintubesorplates(DNAstable)

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Data

SuccessfulPCRafterlongtermstorageinDNAstable:

Figure1.Aliquots(1ng)ofpDNA,eitherstoredinDNAstable(DS)orunprotected(NP)for26monthsatambienttemperature(RT)orat60°Cunderacceleratedagingconditions(equivalentto30yearsofroomtemperaturestorage)wereanalyzedbyPCRamplificationusingpUC19specificprimersgeneratinga490bpamplicon.ReferenceDNAsamples(1ng)werestoredat-20°C(control).

StabilityinLiquid-to-Dry(LD)Format:

Figure2.Twodifferentaliquots(20ng)ofgDNAstoredinDNAstable(DS)atroomtemperaturewithouthumidiycontrolfor3.5yearswereanalyzedbyPCRamplificationusingβ-actinspecificprimersgeneratinga500bpamplicon.ReferenceDNAsample(100ng)wasstoredat-20°C.

RecoveryofgenomicDNAafter26monthsofstorageinDNAstable

Figure3.GenomicDNAsampleswerestoredfor26monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control-20°C;StoredinDNAstable(DS)atroomtemperature(RT);Unprotected(NP)atroomtemperature;StoredinDNAstable(DS)fortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C);5)Unprotected(NP)andstoredfortheequivalentof30yearsatroomtemperature(exposedtoacceleratedagingconditionsat60°C).

qPCRofgenomicDNAstoredfor26monthsinDNAstable

Figure4.qPCRtracesofexperimentsdescribedinFigure3abovewithstandarddeviationdisplayed.

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Specifications

• Productformats:
  • 1.7mltubesand96well-plates(dry)
  • 2mLand10mLbottles(liquid-to-dry)
  • Custom
• Applicationvolume:5to150μl
• Applicationamount:0.001to30μg
• Dryingtime:10min(SpeedVac)toovernight(Laminarhood)
• Recoveryvolume:10to100μl
• Recovery:∼99.9%
• DNAlong-termstorage:30yearsacceleratedaging;∼4years″realtime″
• DNAstabilizationtestingtemperature:Temperature>60°C

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