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主营:DNAgard Saliva, RNAstable, DNAstable, CloneStab, RNAgard, DNAgard
℡ 4000-520-616
℡ 4000-520-616
Biomatrica/DNAstāble™ Plus/ 53091-016/ 2 mL
产品编号:53091-016
市  场 价:¥4800.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$240.00
品      牌: Biomatrica
公      司:Biomatrica, Inc.
公司分类:
Biomatrica/DNAstāble™ Plus/ 53091-016/ 2 mL
商品介绍

StABIlizeandstorepurifiedDNAatRoomTemperature

  • NextgenerationliquidpreservativeforpurifiedDNA–atalltemperatures
  • LiquidformatoptioncompatIBLewithautomationandsamplehandlingworkflows
  • RecoverhighamountsofDNAandanalyzeinstantlywithoutfurtherpurification
  • Compatiblewithmostdownstreamapplications,e.g.,PCR,RT-PCR,SNPanalysis,DNAsequencing,etc.
  • Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement

Product

DNAstāble™Plusenablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatroomtemperatureinliquidandliquid-to-dryformats.ItoffersflexibilitywithThermostabilityatextremetemperatures,andtheabilitytohandlehighconcentrationsofDNA.Recoveryfromthedrymatrixisachievedwithinminutesbysimplyaddingwater.YourDNAsampleisreadyfordownstreamanalysiswithouttheneedforanyadditionalpurificationsteps.

PropertiesBiomatricaUnprotectedColdChain
Yield
Stability
QualityofAmplification
LowConcentrationDNA
ShortTermStorage<1>
LongTermStorage>1year
ShippingRobustness
CostEffectiveness

DNAstabilizationcanbeachievedintwoformats:

  1. LiquidStabilization(short-term)
  2. Liquid-to-Dry(LD)Stabilization(long-term)

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Data

StabilityinLiquidFormat:

Figure1:ThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat45°Cinliquidformatcomparedtounprotectedsamples(TE;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.

StabilityinLiquid-to-Dry(LD)Format:

Figure2:Stabilityfor3yearsat60°CSamples1-3and10-12aboverepresentDNAstoredinTEbufferfor3yearsat4°Cinliquidformat(positivecontrol).Theremainingsampleswerestoreddryat60°Cfor3years.Samples4-9represent50ng/µLDNAstoredineitherDNAstablePlus(4-6)orTE(7-9).Samples13-18represent5ng/µLDNAstoredineitherDNAstablePlus(13-15)orTE(16-18).100ngofthetriplicatesamplesperconditionwererunona1%agarosegelstainedwithethidiumbromide.

Figure3:Stabilityfor6monthsat85°CThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat85°Cinliquid-to-dryformatcomparedtounprotectedsamples(NP;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.

Figure4:Long-rangePCRassaysusingDNAsamplesstoredfor3yearsinDNAstablePlussamples1-3aboverepresentDNAstoredfor3yearsinTEbufferat4°Cinliquidformatwhiletherestofthesampleswerestoreddryat60°Cfor3yearsat50ng/µL.Samples4-6representDNAstoredinDNAstablePlus,whilesamples7-9representDNAstoredinTEbuffer.100ngofrehydratedDNAfromtriplicatesampleswereamplifiedusingKODXtreme™HotStartPCRkit(Novagen)with7.5kbhumanβ-globinprimers.

TechnicalData:

  • DownstreamAnalysis(qPCR)ofgenomicDNAstoreddryfor6monthsatvarious“STRESS”conditions.
  • DNAstablePlusprotectsgenomicDNAunderextremestressconditionsat+85°CinLDformat.
  • DNAstablePlusdoesnotcauseanyinterferencewithdownstreamanalysis.
  • DNArecoveredfromsamplesprotectedwithDNAstablePlus(stressedat60°Cor85°C)aresimilartoDNArecoveredfromstoredat4°C.

Figure5:HumangenomicDNAsampleswerestoredfor6monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control4°C;StoredinDNAstablePlusat60°C;StoredinDNAstablePlusat85°C;Storedunprotected(TE)at60°C;andStoredunprotected(TE)at85°C(noDNArecovered).Thecontrolwasaliquidwhereasallothersampleswererecoveredfromadriedstate.

Figure6:qPCRtracesofDNArecoveryexperiments,describedaboveinFigure5.Representativetracesfromtriplicatesamplesareshown.

Figure7:DNArecoveredafterstorageat+85°Cfor3monthsindriedformat.SamplesprotectedwithDNAstablePlus(DSP)arerecoveredsimilarto-20°Ccontrols.However,non-protected(NP)samplesandcompetition“G”shownoDNArecovery.

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Specifications

  • ProductFormats:2mland10mlLiquidReagents
  • Applicationvolume:1:4(vol:vol)ofstabilizer:liquidDNA
  • Applicationamount:0.001to250µg
  • Dryingtime:10min(SpeedVac)toovernight(Laminarhood)
  • Recoveryvolume:10to200µl
  • Recovery:~99.9%
  • DNAstorageinliquidatRT:1year(acceleratedstudies,6monthsforplasmidDNA)
  • DNAstorageindriedformatatRT:Atleast40years(acceleratedstudies)

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