Biomatrica/DNAstāble™ Plus/ 53091-016/ 2 mL,Biomatrica,,Biomatrica,Biomatrica Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Biomatrica/DNAstāble™ Plus/ 53091-016/ 2 mL

产品编号：53091-016

市场价：¥4800.00

场地：美国(厂家直采)

产品分类：分子类>二代测序>核酸常温运输>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$240.00

品牌： Biomatrica

公司：Biomatrica, Inc.

公司分类：

商品介绍

StABIlizeandstorepurifiedDNAatRoomTemperature

NextgenerationliquidpreservativeforpurifiedDNA–atalltemperatures
LiquidformatoptioncompatIBLewithautomationandsamplehandlingworkflows
RecoverhighamountsofDNAandanalyzeinstantlywithoutfurtherpurification
Compatiblewithmostdownstreamapplications,e.g.,PCR,RT-PCR,SNPanalysis,DNAsequencing,etc.
Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement

Product

DNAstāble™Plusenablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatroomtemperatureinliquidandliquid-to-dryformats.ItoffersflexibilitywithThermostabilityatextremetemperatures,andtheabilitytohandlehighconcentrationsofDNA.Recoveryfromthedrymatrixisachievedwithinminutesbysimplyaddingwater.YourDNAsampleisreadyfordownstreamanalysiswithouttheneedforanyadditionalpurificationsteps.

Properties	Biomatrica	Unprotected	ColdChain
Yield
Stability
QualityofAmplification
LowConcentrationDNA
ShortTermStorage<1>
LongTermStorage>1year
ShippingRobustness
CostEffectiveness

DNAstabilizationcanbeachievedintwoformats:

LiquidStabilization(short-term)
Liquid-to-Dry(LD)Stabilization(long-term)

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Data

StabilityinLiquidFormat:

Figure1:ThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat45°Cinliquidformatcomparedtounprotectedsamples(TE;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.

StabilityinLiquid-to-Dry(LD)Format:

Figure2:Stabilityfor3yearsat60°CSamples1-3and10-12aboverepresentDNAstoredinTEbufferfor3yearsat4°Cinliquidformat(positivecontrol).Theremainingsampleswerestoreddryat60°Cfor3years.Samples4-9represent50ng/µLDNAstoredineitherDNAstablePlus(4-6)orTE(7-9).Samples13-18represent5ng/µLDNAstoredineitherDNAstablePlus(13-15)orTE(16-18).100ngofthetriplicatesamplesperconditionwererunona1%agarosegelstainedwithethidiumbromide.

Figure3:Stabilityfor6monthsat85°CThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat85°Cinliquid-to-dryformatcomparedtounprotectedsamples(NP;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.

Figure4:Long-rangePCRassaysusingDNAsamplesstoredfor3yearsinDNAstablePlussamples1-3aboverepresentDNAstoredfor3yearsinTEbufferat4°Cinliquidformatwhiletherestofthesampleswerestoreddryat60°Cfor3yearsat50ng/µL.Samples4-6representDNAstoredinDNAstablePlus,whilesamples7-9representDNAstoredinTEbuffer.100ngofrehydratedDNAfromtriplicatesampleswereamplifiedusingKODXtreme™HotStartPCRkit(Novagen)with7.5kbhumanβ-globinprimers.

TechnicalData:

DownstreamAnalysis(qPCR)ofgenomicDNAstoreddryfor6monthsatvarious“STRESS”conditions.
DNAstablePlusprotectsgenomicDNAunderextremestressconditionsat+85°CinLDformat.
DNAstablePlusdoesnotcauseanyinterferencewithdownstreamanalysis.
DNArecoveredfromsamplesprotectedwithDNAstablePlus(stressedat60°Cor85°C)aresimilartoDNArecoveredfromstoredat4°C.

Figure5:HumangenomicDNAsampleswerestoredfor6monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control4°C;StoredinDNAstablePlusat60°C;StoredinDNAstablePlusat85°C;Storedunprotected(TE)at60°C;andStoredunprotected(TE)at85°C(noDNArecovered).Thecontrolwasaliquidwhereasallothersampleswererecoveredfromadriedstate.

Figure6:qPCRtracesofDNArecoveryexperiments,describedaboveinFigure5.Representativetracesfromtriplicatesamplesareshown.

Figure7:DNArecoveredafterstorageat+85°Cfor3monthsindriedformat.SamplesprotectedwithDNAstablePlus(DSP)arerecoveredsimilarto-20°Ccontrols.However,non-protected(NP)samplesandcompetition“G”shownoDNArecovery.

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