StABIlizeandstoreRNAatAmbientTemperatures

• PreservesRNAatambienttemperatureswithoutdegradation
• EliminatestheneedtostoreRNAinfreezersortoshipondryice
• ConcentratesRNAmoreeffectivelythanothermethods
• CompatIBLewithmostdownstreamapplications

Overview

RNAstable™andRNAstable™LDallowsyoutopreserveandshipyourtotalRNA,mRNA,andmiRNAforupto12yearsatambienttemperatures*.Theyprovideacost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement.Moreover,theyeliminatesamplelossduetofreeze/thawcycles.WhenyouarereadytouseyourRNAsamples,youcanfullyrecovertheminminuteswithoutanyadditionalpurificationsteps.

RNAstableisavailableintwoconvenientformats:

• RNAstable:providedpre-driedin1.5mlmicrofugetubesand96-wellplatestowhichhydratedRNAcanbeadded.
• RNAstableLD:aliquidreagentwhichcanbeaddeddirectlytoRNAsamplesinyourowntubes,multi-wellplates,orothercontainers

ProductData

Figure1:RNAstableprotectstotalRNA,mRNA,miRNA.Samplescanbesafelystoredfor12yearsatambienttemperatureandprotectedfromdegradation.

Figure2:Successfulgeneexpressionanalysisonmicroarray.

Figure3:(A)AliquotsoftotalRNAwerestoredfor29monthsateither-80°CoratroomtemperatureprotectedinRNAstableorunprotected.RNAintegritywasassessedusingagarosegelelectrophoresisandbioanalyzeranalysis(e.g.RINscore).(B)Aliquotsofthestoredsampleswerequantifiedusingthe18SrRNAgeneinaOne-StepRT-PCRreaction.Threesampleswereamplifiedforeachstorageconditionandtheresultsfromthe9reactionsareoverlaidonthegraph.(C)Recoverylevels(pg)fortotalRNAstoredunderidenticalconditions.

Specifications

• Productformats:
  • Pre-driedin1.5mlmicrofugetubesand96-wellplates(RNAstable)
  • Liquidaliquotsof2mland10ml(RNAstableLD)
• Applicationvolume:100μl
• Applicationamount:0.01to100µg
• Dryingtime:Overnight(laminarflowhood);0.5to1.5hourswithvacuumconcentratorsystem(speedVac®)
• Recoveryvolume:10to100μl
• Recovery:∼99.9%
• DNAstorageatroomtemperature:12yearsacceleratedaging;3.5years“realtime”

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